A spirited second half effort went unrewarded for Blackpool, as Paul Ince's luckless side fell at the first hurdle of this season's FA Cup.
Tom Barkhuizen's stunning goal levelled matters on the stroke of half-time, but Pool were constantly thwarted by Andy Longeran in the Bolton goal, as they searched for a second equaliser.
David Ngog's fine strike had put the hosts ahead, and Jermaine Beckford restored their lead, but Blackpool's performance will give supporters hope.
Chris Basham saw his glancing header saved late on and Thomas Ince came close on more than one occasion, but their efforts proved to be in vain at The Reebok Stadium.
Ince handed Barkhuizen a rare chance, one which is grabbed with both hands, whilst Basham returned to face his former club.
Barkhuizen was involved just a minute in, he followed up a mazy run by setting up Angel Martinez, who narrowly drilled wide from long range.
The early pressure wasn't just Pool's however, as Beckford volleyed a presentable opportunity wide of the mark for Bolton.
Next former-Blackpool man Alex Baptiste tested Matt Gilks, whilst Andre Moritz couldn't turn in the rebound.
Bolton's pressure was rewarded after ten minutes when Ngog was given space to run into, before firing into the top corner from outside the box.
The hosts were looking threatening, next Beckford shot at Gilks after shrugging off Craig Cathcart.
The goalkeeper was then on hand to block a Moritz effort, before Cathcart threw himself at Beckford's rebound.
However, for all their chances, the hosts were stunned on the stroke of half-time.
Barkhuizen cut inside onto his left foot and hit a stunning strike into the top corner to bring his side level, despite momentary confusion regarding the linesman's flag.
Half Time: Bolton 1-1 Blackpool
After the break Blackpool were the better side and Angel was inches away from putting Pool ahead, before a quick Bolton breakaway proved deadly.
Ngog broke down the left and squared for Beckford to slide in and put his side back in front, as the striker scored at this stage of the FA Cup for the fifth successive season - for a fifth different team.
The game had now become stretched, next Chris Eagles bent an effort narrowly wide for Bolton, but it was far from one-way traffic.
Goal scorer Barkhuizen was then forced off with a knock and was replaced by Nathan Eccleston.
With 20 minutes remaining it was all Blackpool, who were giving everything in search for an equaliser.
Ince produced a fine bit of skill to beat his man, before bending a deflected effort inches wide. From the resulting corner Cathcart fire well over the bar.
Ricardo Fuller was next to try his luck, seeing his raking effort palmed away by Andy Lonergan, before an offside Basham headed against the post.
At the other end substitute Chung-Yong Lee rattled the bar with Gilks beaten.
With ten minutes remaining Blackpool went for it and put Michael Chopra on in place of Angel.
Ince then produced a tantalising cross, which was met by a glancing Basham header, only to be kept out by a fine Lonergan save.
Jack Robinson was next to be foiled by the experienced stopper.
As the game entered stoppage time Fuller's header was kept out by his own man, Gary MacKenzie, before Gilks saved from Neil Danns.
Basham's one-two wish Neal Bishop nearly wielded a last-gasp equaliser, but the midfielder fired over and with that went Blackpool's hopes in this season's FA Cup.
Full Time: Bolton 2-1 Blackpool
By Mark Ridgway