Barry Ferguson and Tom Barkhuizen both played the full 90 minutes for Fleetwood Town on Saturday as they lost 1-0 at Dagenham & Redbridge.
Jake Caprice, on-loan at Dagenham, was not involved as he saw his new team-mate Sam Williams dispatch a 90th minute penalty.
Adda Djeziri was not in Scunthorpe's squad after his move last week, but watched his new side go down 4-0 at high-flying Doncaster Rovers.
Elsewhere, Liam Tomsett and Louis Almond were up against each other as Hyde and Barrow played out a 1-1 draw in the FA Trophy.
Tomsett was an unused substitute for Hyde, but Almond played the full 90 minutes at Ewen Fields.
Nathan Eccleston's time at Tranmere has come to an end and the striker has now returned to Bloomfield Road.